Fluorescence & Photoluminescence
Skylark Lasers understands the importance of high spectral and spatial precision in photoluminescence applications. Fluorescence, in the most rigorous sense, is the emission of light from an electron excited into one of its singlet states within the material, commonly a very fast emission following excitation, whereas phosphorescence is the emission of light from the triplet state, causing a slower and more delayed emission of light. With this understanding, Skylark Lasers offers single frequency continuous wave laser sources designed to provide high spectral and spatial precision for various photoluminescence applications.
Our single frequency continuous wave laser sources offer unrivalled wavelength stability and narrow linewidth, resulting in higher spectral and spatial precision for fluorescence imaging. Our visible lasers at 532 and 640 nm as well as our UV lasers at 320 and 349 nm provide high spectral and spatial precision for direct fluorescence imaging of aromatic amino acids and DNA quantitation. Our lasers are designed for integration over a range of wavelengths within a small footprint, making them easy to use and integrate into fluorescence imaging systems.
Photoluminescence is a general term covering the two light emitting mechanisms of fluorescence and phosphorescence. In the most rigorous sense, fluorescence is the emission of light from an electron excited into one of its singlet states within the material - commonly a very fast emission following excitation - whereas phosphorescence is the emission of light from the triplet state - causing a slower and more delayed emission of light.
These terms are often not used in this specific way and generally fluorescence can be thought of as a fast process of light emission following excitation, often on the nanosecond scale or below, in comparison to the slower phosphorescence, often considered on the microsecond scale or above.
While much photoluminescence is possible with broadband sources, high spectral and spatial precision is required for a wide number of applications, such as confocal microscopy, crystalline defect inspection or dynamic mixtures of fluorochromes and fluorophores.
Many applications make use of fluorescence in conjunction with other measurements, such as Raman, where the ability to use the same excitation source for both techniques’ eases integration and analysis of data. A typical example would be solar cell manufacturing and research, where highly structured surfaces are analyzed using both techniques – fluorescence to examine inherent properties such as carrier lifetime or efficiency, and Raman Microscopy to determine feature homogeneity, for instance.
Skylark Lasers design and manufacture single frequency continuous wave laser sources with unrivalled wavelength stability and narrow linewidths - designed for integration over a range of wavelengths within a small footprint. We currently offer single frequency lasers in the red area of the visible spectrum with the 640 NX, along with the 349 NX in the UV.